Z704164
Sigma
Handbook of ELISPOT: Methods and Protocols
| ISBN-10: | 1-58829469-2 |
| ISBN-13: | 978-1-58829469-2 |
Description
| General description | In this book on ELISPOT, a critical enzyme-linked immunospot assay, experts detail how to design, perform, and analyze these assays. The techniques cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry. |
Properties
| publication info | A. Kalyuzhny, Humana Press, 2005, 336 pp., hard cover |
Table Of Contents
| Table of Contents | PART I. ELISPOT ASSAY SETUP AND DATA ANALYSIS 1. The ELISPOT Assay: A Personal Retrospective 2. Chemistry and Biology of the ELISPOT Assay 3. Membranes and Membrane Plates Used in ELISPOT 4. Standardization and Validation Issues of the ELISPOT Assay 5. A Cell Detachment Solution Can Reduce Background Staining in the ELISPOT Assay 6. Isolation of Subsets of Immune Cells 7. Image Analysis and Data Management of ELISPOT Assay Results 8. High Resolution as a Key Feature to Perform Accurate ELISPOT Measurements Using Zeiss KS ELISPOT Readers PART II. ELIPSOT ASSAY IN ANIMAL MODELS 9. Improving the Sensitivity of the ELISPOT Analyses of Antigen Specific Cellular Immune Responses in Rhesus Macaques 10. Feline Cytokine ELISPOT: Issues in Assay Development 11. Measuring T-Cell Function in Animal Models of Tuberculosis by ELISPOT 12. Interferon-g ELISPOT Assay for the Quantitative Measurements of Antigen Specific Murine CD8+ T-Cells PART III. ELISPOT ASSAYS IN HUMAN STUDIES 13. Detection of Measles Virus Specific Interferon-g-Secreting T-Cells by ELISPOT 14. Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assay 15. Use of Interferon-g ELISPOT in Monitoring Immune Responses in Humans 16. ELISPOT Determination of Interferon-g T-Cell Frequencies in Patients with Autoimmune Sensorineural Hearing Loss PART IV. MULTIPLEX AND MODIFIED ELISPOT ASSAY FORMATS 17. Dual Color ELISPOT Assay for Analyzing Cytokine Balance 18. Simultaneous Detection of Multiple Cytokines in ELISPOT Assays 19. Fluorospot Assay: Methodological Analysis 20. A Gel Based Dual Antibody Capture and Detection Method for Assaying of Extracellular Cytokine Secretion: EliCell Index |
